Eco-Friendly Sol-Gel Synthesis of Silver Nanoparticles using Morinda citrifolia Leaf Extract and PVP: Structural, Morphological and Antibacterial Properties
DOI:
https://doi.org/10.25077/jif.18.2.129-144.2026Keywords:
Antibacterial, Eco-friendly sol-gel, Morinda citrifolia leaf extract, Polyvinylpyrrolidone, Silver nanoparticlesAbstract
Silver nanoparticles (AgNPs) have strong potential as antibacterial agents, with effectiveness highly influenced by particle-size stability. This study synthesized AgNPs via a green sol-gel method using noni (Morinda citrifolia) leaf extract as a natural reducing agent and polyvinylpyrrolidone (PVP) as a capping agent. PVP concentrations of 0.5, 1.0, and 1.5 g were mixed with 25 mL noni extract and 25 mL double-distilled water. Characterization using UV–vis, XRD, FE-SEM/EDX, and FTIR showed decreasing UV–vis absorption peaks of 433, 412, 368 nm with increasing PVP content. XRD confirmed a face-centered cubic (FCC) crystal structure with >80% similarity to standard Ag patterns. FE-SEM/EDX indicated the presence of Ag, C, and O elements, and particle sizes remained stable at 16.8, 18.8, and 18.4 nm. The 0.5 g PVP sample produced the smallest particles and showed dominant O–H and C–H functional groups. It exhibited the strongest antibacterial activity, with inhibition zones of 12.5 mm (E. coli) and 12.0 mm (S. aureus). These results demonstrate that eco-friendly sol-gel-synthesized AgNPs using noni leaf extract and PVP exhibit promising antibacterial activity, positioning them as potential candidates for further development as antibacterial materials.
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Abid, N., Khan, A. M., Shujait, S., Chaudhary, K., Ikram, M., Imran, M., Haider, J., Khan, M., Khan, Q., & Maqbool, M. (2022). Synthesis of nanomaterials using various top-down and bottom-up approaches, influencing factors, advantages, and disadvantages: A review. Advances in Colloid and Interface Science, 300, 102597. https://doi.org/10.1016/j.cis.2021.102597
Adrianto, N., Panre, A. M., Istiqomah, N. I., Riswan, M., Apriliani, F., & Suharyadi, E. (2022). Localized surface plasmon resonance properties of green synthesized silver nanoparticles. Nano-Structures & Nano-Objects, 31, 100895. https://doi.org/10.1016/j.nanoso.2022.100895
Agustina, T. E., Handayani, W., & Imawan, C. (2021). The UV-vis spectrum analysis from silver nanoparticles synthesized using Diospyros maritima blume. leaves extract. Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020), 411–419. https://doi.org/10.2991/absr.k.210621.070
Akintelu, S. A., Folorunso, A. S., Oyebamiji, A. K., & Olugbeko, S. C. (2021). Mosquito repellent and antibacterial efficiency of facile and low-cost silver nanoparticles synthesized using the leaf extract of Morinda citrifolia. Plasmonics, 16(5), 1645–1656. https://doi.org/10.1007/s11468-021-01428-3
Al-Mahmud, M. R., Hossain Shishir, M. K., Ahmed, S., Tabassum, S., Islam Sadia, S., Haque Sachchu, M. M., Tanbin Tama, R., Rahim Miah, A., & Ashraful Alam, M. (2024). Stoichiometry crystallographic phase analysis and crystallinity integration of silver nanoparticles: A rietveld refinement study. Journal of Crystal Growth, 643, 127815. https://doi.org/10.1016/j.jcrysgro.2024.127815
Ali, M. H., Azad, M. A. K., Khan, K. A., Rahman, M. O., Chakma, U., & Kumer, A. (2023). Analysis of crystallographic structures and properties of silver nanoparticles synthesized using PKL extract and nanoscale characterization techniques. ACS Omega, 8(31), 28133–28142. https://doi.org/10.1021/acsomega.3c01261
AlQurashi, D. M., AlQurashi, T. F., Alam, R. I., Shaikh, S., & Tarkistani, M. A. M. (2025). Advanced nanoparticles in combating antibiotic resistance: Current innovations and future directions. Journal of Nanotheranostics, 6(2), 9. https://doi.org/10.3390/jnt6020009
Anas, I. Z., & Yotenka, R. (2025). Analisis cluster luas lahan panen tanaman biofarma di Indonesia tahun 2022. Emerging Statistics and Data Science Journal, 3(1), 485–497. https://doi.org/10.20885/esds.vol3.iss.1.art5
Arif, M. S., Ulfiya, R., Erwin, & Panggabean, A. S. (2021). Synthesis silver nanoparticles using trisodium citrate and development in analysis method. The 6TH International Conference on Basic Sciences 2020 (ICBS 2020), 050007. https://doi.org/10.1063/5.0059493
Badanayak, P., Vastrad, J. V, & Author, C. (2021). Sol-gel process for synthesis of nanoparticles and applications thereof. The Pharma Innovation Journal, 10(8), 1023–1027. https://www.thepharmajournal.com
Bala, A., & Rani, G. (2020). A review on phytosynthesis, affecting factors and characterization techniques of silver nanoparticles designed by green approach. International Nano Letters, 10(3), 159–176. https://doi.org/10.1007/s40089-020-00309-7
Basak, M., Rahman, M. L., Ahmed, M. F., Biswas, B., & Sharmin, N. (2022). The use of X-ray diffraction peak profile analysis to determine the structural parameters of cobalt ferrite nanoparticles using Debye-Scherrer, Williamson-Hall, Halder-Wagner and Size-strain plot: Different precipitating agent approach. Journal of Alloys and Compounds, 895, 162694. https://doi.org/10.1016/j.jallcom.2021.162694
Bokov, D., Turki Jalil, A., Chupradit, S., Suksatan, W., Javed Ansari, M., Shewael, I. H., Valiev, G. H., & Kianfar, E. (2021). Nanomaterial by sol‐gel method: Synthesis and application. Advances in Materials Science and Engineering, 2021(1). https://doi.org/10.1155/2021/5102014
Chavda, M., Manani, L., Chandarana, C., & Chavda, P. (2025). A review on noni: Insights into botany, ethnopharmacology, phytochemistry, and commercial prospects. International Journal of Pharmacognosy, 12(2), 100–112. https://doi.org/10.13040/IJPSR.0975-8232.IJP.12(2).100-12
Chen, C., Zhou, H., Ma, Y., Dai, Q., & Tang, Z. (2023). Celebrating 20 years of NCNST: Innovation in nanoscience and nanotechnology. ACS Nano. https://doi.org/10.1021/acsnano.3c06711
Clinical and Laboratory Standards Institute (CLSI). (2020). Performance Standards for Antimicrobial Susceptibility Testing. In Journal of Clinical Microbiology (30th ed.). CLSI. https://doi.org/10.1128/JCM.01864-19
de Souza, C. C., Ramos, G. Q., Katak, R. de M., Muniz, V. A., Roque, R. A., Ferreira, N. S., Matos, R. S., Xing, Y., & Filho, H. D. F. (2024). Eco-friendly synthesis of silver nanoparticles via Cassava starch: structural analysis and biocidal applications against aedes aegypti and pathogenic bacteria. Journal of Sol-Gel Science and Technology. https://doi.org/10.1007/s10971-024-06606-3
Girma, A., Alamnie, G., Bekele, T., Mebratie, G., Mekuye, B., Abera, B., Workineh, D., Tabor, A., & Jufar, D. (2024). Green-synthesised silver nanoparticles: Antibacterial activity and alternative mechanisms of action to combat multidrug-resistant bacterial pathogens: A systematic literature review. Green Chemistry Letters and Reviews, 17(1). https://doi.org/10.1080/17518253.2024.2412601
Ibrahim, N. H., Taha, G. M., Hagaggi, N. S. A., & Moghazy, M. A. (2024). Green synthesis of silver nanoparticles and its environmental sensor ability to some heavy metals. BMC Chemistry, 18(1), 7. https://doi.org/10.1186/s13065-023-01105-y
Ider, M., Abderrafi, K., Eddahbi, A., Ouaskit, S., & Kassiba, A. (2017). Silver metallic nanoparticles with surface plasmon resonance: Synthesis and characterizations. Journal of Cluster Science, 28(3), 1051–1069. https://doi.org/10.1007/s10876-016-1080-1
Javed, R., Zia, M., Naz, S., Aisida, S. O., Ain, N. ul, & Ao, Q. (2020). Role of capping agents in the application of nanoparticles in biomedicine and environmental remediation: Recent trends and future prospects. Journal of Nanobiotechnology, 18(1), 172. https://doi.org/10.1186/s12951-020-00704-4
Khan, M. R., Urmi, M. A., Kamaraj, C., Malafaia, G., Ragavendran, C., & Rahman, M. M. (2023). Green synthesis of silver nanoparticles with its bioactivity, toxicity and environmental applications: A comprehensive literature review. Environmental Nanotechnology, Monitoring & Management, 20, 100872. https://doi.org/10.1016/j.enmm.2023.100872
Menichetti, A., Mavridi-Printezi, A., Mordini, D., & Montalti, M. (2023). Effect of size, shape and surface functionalization on the antibacterial activity of silver nanoparticles. Journal of Functional Biomaterials, 14(5), 244. https://doi.org/10.3390/jfb14050244
Mistry, H., Thakor, R., Patil, C., Trivedi, J., & Bariya, H. (2021). Biogenically proficient synthesis and characterization of silver nanoparticles employing marine procured fungi Aspergillus brunneoviolaceus along with their antibacterial and antioxidative potency. Biotechnology Letters, 43(1), 307–316. https://doi.org/10.1007/s10529-020-03008-7
Mohrig, J. R., Hammond, C. N., & Schatz, P. F. (2010). Techniques in Organic Chemistry. Clancy Marshall.
Morales-Lozoya, V., Espinoza-Gómez, H., Z. Flores-López, L., Sotelo-Barrera, E. L., Núñez-Rivera, A., Cadena-Nava, R. D., Alonso-Nuñez, G., & Rivero, I. A. (2021). Study of the effect of the different parts of Morinda citrifolia L. (noni) on the green synthesis of silver nanoparticles and their antibacterial activity. Applied Surface Science, 537, 147855. https://doi.org/10.1016/j.apsusc.2020.147855
More, P. R., Pandit, S., Filippis, A. De, Franci, G., Mijakovic, I., & Galdiero, M. (2023). Silver nanoparticles: Bactericidal and mechanistic approach against drug resistant pathogens. Microorganisms, 11(2), 369. https://doi.org/10.3390/microorganisms11020369
Munir, T., Mahmood, A., Imran, M., Sohail, A., Fakhar-e-Alam, M., Sharif, M., Masood, T., Bajwa, S. Z., Shafiq, F., & Latif, S. (2021).
Quantitative analysis of glucose by using (PVP and MA) capped silver nanoparticles for biosensing applications. Physica B: Condensed Matter, 602, 412564. https://doi.org/10.1016/j.physb.2020.412564
Mustapha, S., Tijani, J. O., Ndamitso, M. M., Abdulkareem, A. S., Shuaib, D. T., Amigun, A. T., & Abubakar, H. L. (2021). Facile synthesis and characterization of TiO2 nanoparticles: X-ray peak profile analysis using Williamson–Hall and Debye–Scherrer methods. International Nano Letters, 11(3), 241–261. https://doi.org/10.1007/s40089-021-00338-w
Nagalingam, M., Rajeshkumar, S., Balu, S. K., Tharani, M., & Arunachalam, K. (2022). Anticancer and antioxidant activity of Morinda citrifolia leaf mediated selenium nanoparticles. Journal of Nanomaterials, 2022(1). https://doi.org/10.1155/2022/2155772
Nandiyanto, A. B. D., Oktiani, R., & Ragadhita, R. (2019). How to read and interpret FTIR spectroscope of organic material. Indonesian Journal of Science and Technology, 4(1), 97. https://doi.org/10.17509/ijost.v4i1.15806
Nethi, S. K., Mukherjee, A., & Mukherjee, S. (2021). Biosynthesized Gold and Silver Nanoparticles in Cancer Theranostics. In Handbook of Nanomaterials and Nanocomposites for Energy and Environmental Applications (pp. 759–773). Springer International Publishing. https://doi.org/10.1007/978-3-030-36268-3_29
Neto, F. N. S., Morais, L. A., Gorup, L. F., Ribeiro, L. S., Martins, T. J., Hosida, T. Y., Francatto, P., Barbosa, D. B., Camargo, E. R., & Delbem, A. C. B. (2023). Facile synthesis of PVP-coated silver nanoparticles and evaluation of their physicochemical, antimicrobial and toxic activity. Colloids and Interfaces, 7(4), 66. https://doi.org/10.3390/colloids7040066
Onu, B. U. (2024). Analysis of the phytochemical components of noni (Morinda citrifolia) leaves and stem. SSRN Electronic Journal. https://doi.org/10.2139/ssrn.4752639
Patil, R. B., & Chougale, A. D. (2021). Analytical methods for the identification and characterization of silver nanoparticles: A brief review. Materials Today: Proceedings, 47, 5520–5532. https://doi.org/10.1016/j.matpr.2021.03.384
Putri, S. A., Nur Shadrina, A. A., Julaeha, E., & Kurnia, D. (2023). Potential nevadensin from Ocimum basilicum as antibacterial agent against Streptococcus mutans: In vitro and in silico studies. Combinatorial Chemistry & High Throughput Screening, 26(9), 1746–1754. https://doi.org/10.2174/1386207325666220930122813
Rather, M. A., Gupta, K., Bardhan, P., Borah, M., Sarkar, A., Eldiehy, K. S. H., Bhuyan, S., & Mandal, M. (2021). Microbial biofilm: A matter of grave concern for human health and food industry. Journal of Basic Microbiology, 61(5), 380–395. https://doi.org/10.1002/jobm.202000678
Rathod, S. M., Gaikwad, S. V., Gore, S. K., Tumberphale, U. B., Shaikh, S. F., Ubaidullah, M., Pandit, B., & Jadhav, S. S. (2023). Ni–Ag ferrites synthesized by sol gel route using aloe vera extract as a solvent: Enhancement in structural, dielectric, magnetic and optical properties. Physica B: Condensed Matter, 661, 414944. https://doi.org/10.1016/j.physb.2023.414944
Riyatun, R., Kusumaningsih, T., Supriyanto, A., & Purnama, B. (2023). Characteristics of the microstructure, magnetic and antibacterial properties of silver-substituted cobalt ferrite nanoparticles from the sol-gel method. Kuwait Journal of Science, 50(4), 569–574. https://doi.org/10.1016/j.kjs.2023.04.001
Sarkar, B., Bhattacharya, P., Yen Chen, C., Maity, J. P., & Biswas, T. (2022). A comprehensive characterization and therapeutic properties in ripened Noni fruits (Morinda citrifolia L.). International Journal of Experimental Research and Review, 29, 10–32. https://doi.org/10.52756/ijerr.2022.v29.002
Simsir, H., Eltugral, N., & Karagoz, S. (2019). The role of capping agents in the fabrication of nano-silver-decorated hydrothermal carbons. Journal of Environmental Chemical Engineering, 7(5), 103415. https://doi.org/10.1016/j.jece.2019.103415
Sreelekha, E., George, B., Shyam, A., Sajina, N., & Mathew, B. (2021). A comparative study on the synthesis, characterization, and antioxidant activity of green and chemically synthesized silver nanoparticles. BioNanoScience, 11(2), 489–496. https://doi.org/10.1007/s12668-021-00824-7
Vanlalveni, C., Lallianrawna, S., Biswas, A., Selvaraj, M., Changmai, B., & Rokhum, S. L. (2021). Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: a review of recent literature. RSC Advances, 11(5), 2804–2837. https://doi.org/10.1039/d0ra09941d
Venkatraman, G., Mohan, P. S., Abdul-Rahman, P. S., Sonsudin, F., Muttiah, B., Hirad, A. H., Alarfaj, A. A., & Wang, S. (2024). Morinda citrifolia leaf assisted synthesis of ZnO decorated Ag bio-nanocomposites for in-vitro cytotoxicity, antimicrobial and anticancer applications. Bioprocess and Biosystems Engineering, 47(8), 1213–1226. https://doi.org/10.1007/s00449-024-02995-5
Yasser, M., & Asfar, A. M. I. A. (2021). Karakterisasi uji kestabilan nanopartikel perak-ekstrak daun sirih hijau menggunakan spektroskopi UV-vis. Seminar Nasional Penelitian & Pengabdian Kepada Masyarakat (SNP2M), 29–32.
Zein, R., Alghoraibi, I., Soukkarieh, C., Ismail, M. T., & Alahmad, A. (2022). Influence of polyvinylpyrrolidone concentration on properties and anti-bacterial activity of green synthesized silver nanoparticles. Micromachines, 13(5), 777. https://doi.org/10.3390/mi13050777
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